Impedimetric Response of a Label-Free Genosensor Prepared on a 3-Mercaptopropionic Acid Capped Gallium Selenide NanocrystalModified Gold Electrode
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Date
2011Author
Ndangili, Peter M.
Jijana, Abongile N.
Olowu, Rasaq A.
Mailu, Stephen N.
Ngece, Fanelwa R.
Williams, Avril
Waryo, Tesfaye T.
Baker, Priscilla G.L.
Iwuoha, Emmanuel I.
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Biocompatible and water soluble 3-mercaptopropionic acid cappedgallium selenide nanocrystals were synthesized from hydrated gallium (III) perchlorate and selenide ions. The nanocrystals were non-fluorescent but showed a sharp UV-vis absorption maximum at 260 nm.Transmission electron micrographs showed the formation of high quality non-aggregated particles with an average diameter of 65 nm. Gold electrode modified with the capped nanocrystals was used as a platform for impedimetric genosensing using NH2-5′-CCCACCGGTCCTTCATGTTC-3′ (probe) and 5′-GAACATGAAGGA CCGGTGGG-3′ (target) oligonucleotide sequences. The target oliginucleotide sequence is a component of5-enolpyruvylshikimate-3-phosphate synthase, a common vector gene in glyphosate resistant transgenic plants. The impedimetric genosensor exhibited high sensitivity towards the target DNA (sensitivity = 11.61 Ω/nM) with a detection limit of 0.66 nM (3s, n = 8). The genosensor was able to discriminate between complementary, non-complementary and 3-base mismatched target sequences and maintained 87 % of its response towards the target DNA after one month.