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dc.contributor.authorNgigi, Anastasiah
dc.contributor.authorGetenga, Zachary
dc.contributor.authorBoga, Hamadi
dc.contributor.authorNdalut, Paul
dc.date.accessioned2019-07-16T05:42:24Z
dc.date.available2019-07-16T05:42:24Z
dc.date.issued2011
dc.identifier.issn0277-2248
dc.identifier.urihttp://ir.mksu.ac.ke/handle/123456780/4603
dc.description.abstractThe phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used alone or in a broad range of herbicide formulations. Its degradation in sugarcane-cultivated soils which have been impacted by the herbicide through repeated applications was studied. Liquid culture experiments with diuron as the only carbon source led to the isolation of different bacterial strains capable of degrading diuron. The bacterial species belonging to the genera Bacillus, Vagococcus, and Burkholderia, identified through biochemical and molecular characterization, degraded diuron to different extents. The isolated Bacillus cereus, Vagococcus fluvialis, Burkholderia ambifaria, and Bacillus spp1 degraded diuron by 21%, 25%, 22%, and 19% of the initially applied concentration of 40 mg L1 , respectively, after 35 days of incubation in liquid culture media. Small amounts of 3,4-dichloroaniline and the de-methylated metabolite N-(3,4- dichlorophenyl)-N-methylurea were detected in liquid culture media. The combination of V. fluvialis and B. ambifaria showed an enhanced degradation of up to 30% of the initially applied concentration of 40 mg L1 . Degradation by pure isolates was low (18–25%) compared to the capacities of diuron degradation shown by the bacterial communities (58–74%). This study showed the presence of diuron degraders in sugarcane-cultivated soils impacted by diuron due to repeated applications.en_US
dc.language.isoen_USen_US
dc.publisherTaylor & Francisen_US
dc.subjectdiuronen_US
dc.subjectdegradationen_US
dc.subjectmetabolitesen_US
dc.subjectbacterial isolatesen_US
dc.subjectliquid culturesen_US
dc.titleBiodegradation of phenylurea herbicide diuron by microorganisms from long-term-treated sugarcane-cultivated soils in Kenyaen_US
dc.typeArticleen_US


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