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dc.contributor.authorMathooko, Francis M.
dc.contributor.authorMwaniki, Mercy W.
dc.contributor.authorNakatsuka, Akira
dc.contributor.authorShiomi, Shinjiro
dc.contributor.authorKubo, Yasutaka
dc.contributor.authorInaba, Akitsugu
dc.contributor.authorNakamura, Reinosuke
dc.date.accessioned2019-04-08T08:04:14Z
dc.date.available2019-04-08T08:04:14Z
dc.date.issued1999
dc.identifier.urihttp://ir.mksu.ac.ke/handle/123456780/4253
dc.description.abstractWe investigated the expression pattern of three 1-aminocydopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CSACS1 transcripts which disappeared upon withdrawal of CO2. Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.subjectACC synthaseen_US
dc.subjectCO2 stressen_US
dc.subjectCucumberen_US
dc.subjectCucumis sativusen_US
dc.subjectEthylene biosynthesisen_US
dc.subjectGene expressionen_US
dc.subjectSignal transduction.en_US
dc.titleExpression Characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-AminocycIopropane-l-Carboxylate Synthase Gene Family in Cucumber {Cucumis sativus L.) Fruit under Carbon Dioxide Stressen_US
dc.typeArticleen_US


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